vector pcag dsred2 Search Results


dsred2  (TaKaRa)
95
TaKaRa dsred2
Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing <t>DsRed2</t> presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).
Dsred2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector pcag dsred2  (Addgene inc)
92
Addgene inc vector pcag dsred2
Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing <t>DsRed2</t> presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).
Vector Pcag Dsred2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pdsred2 n1  (TaKaRa)
95
TaKaRa pdsred2 n1
Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing <t>DsRed2</t> presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).
Pdsred2 N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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piggybac transposon vectors (ppb-cag-dsred2)  (Max Planck Florida Institute for Neuroscience)
90
Max Planck Florida Institute for Neuroscience piggybac transposon vectors (ppb-cag-dsred2)
Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing <t>DsRed2</t> presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).
Piggybac Transposon Vectors (Ppb Cag Dsred2), supplied by Max Planck Florida Institute for Neuroscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcag ecfp  (TaKaRa)
86
TaKaRa pcag ecfp
Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing <t>DsRed2</t> presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).
Pcag Ecfp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pecfp n1  (TaKaRa)
86
TaKaRa pecfp n1
Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing <t>DsRed2</t> presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).
Pecfp N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n1 vectors  (Addgene inc)
94
Addgene inc n1 vectors
Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing <t>DsRed2</t> presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).
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mruby lifeact  (Addgene inc)
93
Addgene inc mruby lifeact
Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing <t>DsRed2</t> presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).
Mruby Lifeact, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleofector solution mouse neuron kit  (Lonza)
90
Lonza nucleofector solution mouse neuron kit
Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing <t>DsRed2</t> presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).
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discosoma dsred  (TaKaRa)
86
TaKaRa discosoma dsred
Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing <t>DsRed2</t> presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).
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piggybac transposase (pcag–hypbase)  (Max Planck Florida Institute for Neuroscience)
90
Max Planck Florida Institute for Neuroscience piggybac transposase (pcag–hypbase)
Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing <t>DsRed2</t> presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).
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Image Search Results


Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing DsRed2 presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).

Journal: The Journal of Neuroscience

Article Title: Specific Trans -Synaptic Interaction with Inhibitory Interneuronal Neurexin Underlies Differential Ability of Neuroligins to Induce Functional Inhibitory Synapses

doi: 10.1523/JNEUROSCI.1811-12.2013

Figure Lengend Snippet: Presynaptic overexpression of Nrxn alone does not change inhibitory synaptic transmission. A–C, Effect of postsynaptic overexpression of NLs (NL1, red; NL2: blue) on unitary inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. A, Configuration of dual whole-cell recording (top) and averaged sample uIPSC traces by two presynaptically applied depolarization commands (bottom). B, C, Summary of uIPSC amplitude (B) and connectivity (C). The cell pairs, which consist of untransfected interneurons and GFP-expressing postsynaptic pyramidal neurons, were used as the control. p = 0.014 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of presynaptic overexpression of Nrxns (αNrxn1, light gray; βNrxn1, dark gray) on inhibitory synaptic transmission in hippocampal CA1 pyramidal neurons. D, Configuration of dual whole-cell recording (top), and averaged sample uIPSC traces (bottom). Nrxns were overexpressed in CA1 interneurons by electroporation and postsynaptic response was measured from neighboring untransfected CA1 pyramidal neurons. Summary of uIPSC amplitude (E) and connectivity (F). Controls are neuronal pairs expressing DsRed2 presynaptically. Number in each bar represents the number of synaptically connected cell pairs (B, E) and total number of cell pairs tested (C, F).

Article Snippet: Enhanced green fluorescent protein (EGFP), DsRed2 (Clontech), and TagBFP (Evrogen) were subcloned in the pCAG vector.

Techniques: Over Expression, Transmission Assay, Expressing, Electroporation

NL–Nrxn interaction confers the functional inhibitory synapse formation. A–C, Effect of dual overexpression of NL1AB (red) and βNrxn1 or αNrxn1 in postsynaptic and presynaptic neurons on unitary inhibitory synaptic transmission. A, Configuration of dual whole-cell recording (left top), superimposed fluorescent and Nomarski image (left bottom), and averaged sample uIPSC traces (right). NLs were biolistically transfected in CA1 pyramidal neurons (green), and interneurons, which located within 100 μm from NL-transfected neurons, were transfected Nrxns by electroporation (red). B, C, Summary of uIPSC amplitude (B) and connectivity (C). p = 0.002 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of dual overexpression of NL2A (blue) and βNrxn1 or αNrxn1 in postsynaptic and presynaptic neurons on unitary inhibitory synaptic transmission. D, Averaged sample uIPSC traces. Summary of uIPSC amplitude (E) and connectivity (F). p = 0.00006 by one-way ANOVA, post hoc Dunnett test *p < 0.05. The bar graphs of postsynaptic overexpression of NL1AB and NL2A on uIPSC and connectivity in Figure 5,B and C, are included for a more comprehensive presentation of the finding in the main text. Thirty-one cell pairs expressing GFP and DsRed2 in postsynaptic and presynaptic neurons, and control pairs in Figure 5 were pooled as the control cell pairs (Cntl, black bars).

Journal: The Journal of Neuroscience

Article Title: Specific Trans -Synaptic Interaction with Inhibitory Interneuronal Neurexin Underlies Differential Ability of Neuroligins to Induce Functional Inhibitory Synapses

doi: 10.1523/JNEUROSCI.1811-12.2013

Figure Lengend Snippet: NL–Nrxn interaction confers the functional inhibitory synapse formation. A–C, Effect of dual overexpression of NL1AB (red) and βNrxn1 or αNrxn1 in postsynaptic and presynaptic neurons on unitary inhibitory synaptic transmission. A, Configuration of dual whole-cell recording (left top), superimposed fluorescent and Nomarski image (left bottom), and averaged sample uIPSC traces (right). NLs were biolistically transfected in CA1 pyramidal neurons (green), and interneurons, which located within 100 μm from NL-transfected neurons, were transfected Nrxns by electroporation (red). B, C, Summary of uIPSC amplitude (B) and connectivity (C). p = 0.002 by one-way ANOVA, post hoc Dunnett test *p < 0.05. D–F, Effect of dual overexpression of NL2A (blue) and βNrxn1 or αNrxn1 in postsynaptic and presynaptic neurons on unitary inhibitory synaptic transmission. D, Averaged sample uIPSC traces. Summary of uIPSC amplitude (E) and connectivity (F). p = 0.00006 by one-way ANOVA, post hoc Dunnett test *p < 0.05. The bar graphs of postsynaptic overexpression of NL1AB and NL2A on uIPSC and connectivity in Figure 5,B and C, are included for a more comprehensive presentation of the finding in the main text. Thirty-one cell pairs expressing GFP and DsRed2 in postsynaptic and presynaptic neurons, and control pairs in Figure 5 were pooled as the control cell pairs (Cntl, black bars).

Article Snippet: Enhanced green fluorescent protein (EGFP), DsRed2 (Clontech), and TagBFP (Evrogen) were subcloned in the pCAG vector.

Techniques: Functional Assay, Over Expression, Transmission Assay, Transfection, Electroporation, Expressing